脐带间充质干细胞的内皮分化及其与骨髓间充质干细胞的对比研究

发布时间：2019-04-09 08:57

【摘要】：间充质干细胞(MSCs)作为组织工程的种子细胞具有良好的应用前景。而通过组织工程得到的新生组织的新血管生成越来越成为制约组织工程发展的关键因素。在本次研究中,由脐带沃顿胶质组织(Wharton’s Jelly)分离所得的间充质干细胞(UCMSCs)经内皮细胞分化诱导后可分化成内皮细胞,并首次就其内皮分化能力与骨髓来源间充质干细胞(BMMSCs)进行了较为全面的比较。 UCMSCs与BMMSCs具有相似的梭形成纤维细胞状的细胞形态,流式细胞术分析表明二者均表达典型间充质干细胞标志物且表达水平相当,且碱性磷酸酶活性染色和Von Kossa染色表明二者均具有类似的以成骨分化为典型代表的多向分化能力。然而,UCMSCs比BMMSCs具有更快的增殖速度和更强的增殖能力。经内皮分化诱导后,免疫荧光细胞化学分析和实时定量聚合酶链式反应(Real-Time PCR)分析均表明UCMSCs比BMMSCs表达更高水平的三种内皮细胞特征性标志蛋白;微血管生成能力的检测也表明UCMSCs比BMMSCs具有更强的微血管生成能力;低密度脂蛋白吸收能力的检测表明二者具有相似的低密度脂蛋白吸收能力。以上结果表明脐带间充质干细胞(UCMSCs)比骨髓来源间充质干细胞(BMMSCs)具有更强的内皮细胞分化能力。另外,与骨髓来源间充质干细胞(BMMSCs)相比,由于脐带间充质干细胞(UCMSCs)来源于免疫系统尚未发育完善的新生儿脐带,所以UCMSCs作为组织工程的种子细胞在进行移植后引起免疫排斥机率相对较小,而且由于脐带传统上作为医疗废弃物,所以脐带间充质干细胞(UCMSCs)的来源也具有易获得性。以上这些数据表明,与骨髓来源间充质干细胞(BMMSCs)相比,脐带沃顿胶组织(Wharton’s Jelly)来源的间充质干细胞(UCMSCs)可以成为组织工程中新血管形成及血管损伤相关疾病的治疗中的更理想的细胞来源的选择。
[Abstract]:As seed cells of tissue engineering, mesenchymal stem cells (MSCs) have good application prospects. The neovascularization of new tissue by tissue engineering is becoming the key factor to restrict the development of tissue engineering. In this study, mesenchymal stem cells (UCMSCs) isolated from umbilical cord Wharton glial tissue (Wharton's Jelly) could differentiate into endothelial cells after induced by endothelial cell differentiation. The ability of endothelial differentiation was compared with that of bone marrow derived mesenchymal stem cells (BMMSCs) for the first time. UCMSCs and BMMSCs had similar cell morphology of spindle forming fiber. Flow cytometry analysis showed that both of them expressed typical mesenchymal stem cell markers and expressed at the same level. Alkaline phosphatase activity staining and Von Kossa staining showed that both of them had similar ability of multidirectional differentiation represented by osteogenic differentiation. However, UCMSCs has faster proliferation speed and stronger proliferation ability than BMMSCs. After induction of endothelial differentiation, immunofluorescence cytochemical analysis and real-time quantitative polymerase chain reaction (Real-Time PCR) analysis showed that the expression of UCMSCs was higher than that of BMMSCs in three characteristic marker proteins of endothelial cells. The microangiogenic ability of UCMSCs was also higher than that of BMMSCs, and the ability of LDL absorption was similar to that of LDL. These results suggest that umbilical cord mesenchymal stem cells (UCMSCs) have stronger ability of endothelial cell differentiation than bone marrow derived mesenchymal stem cells (BMMSCs). In addition, compared with bone marrow derived mesenchymal stem cells (BMMSCs), umbilical cord mesenchymal stem cells (UCMSCs) are derived from the umbilical cord of newborn whose immune system is not well developed. Therefore, as the seed cells of tissue engineering, the probability of immune rejection caused by UCMSCs transplantation is relatively small, and the (UCMSCs) source of umbilical cord mesenchymal stem cells is also easy to acquire because the umbilical cord is traditionally used as medical waste. These data suggest that compared with bone marrow derived mesenchymal stem cells (BMMSCs), (UCMSCs) derived from umbilical cord Wharton gum tissue derived mesenchymal stem cells (UCMSCs) may be an ideal cell source for the treatment of new angiogenesis and vascular injury-related diseases in tissue engineering.
【学位授予单位】：汕头大学
【学位级别】：硕士
【学位授予年份】：2008
【分类号】：R329

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