间充质干细胞分化为心肌细胞过程中细胞形态变化与Nkx2.5表达相关性的初步研究
发布时间：2019-05-08 07:01
【摘要】：体外BMSCs分化为心肌细胞过程中,细胞在形态及相关基因的表达上发生明显的变化。本研究以心肌组织裂解液诱导BMSCs,观察分化过程中细胞形态变化,根据心肌细胞发生过程中细胞形态变化规律,选定5个检测点,行PCR检测细胞中Nkx2.5的表达,以探究分化过程中细胞形态变化与相关基因表达规律及其之间的相关性。实验结果表明BMSCs分化为心肌细胞的过程中,细胞形态变化呈现明显规律:由单个大核,胞浆饱满,折光性好的纺锤形;逐渐演变为短杆状;形成突起并连接成网状结构;细胞端相接呈"竹节样"改变;最后形成肌管样结构;诱导前细胞不表达Nkx2.5,诱导过程中随着细胞形态的不断变化Nkx2.5持续表达并逐渐增强。说明间充质干细胞分化为心肌细胞的过程中,Nkx2.5表达相对量的变化与细胞的形态变化可能有一定的相关。
[Abstract]:During the differentiation of BMSCs into cardiomyocytes in vitro, there were obvious changes in morphology and expression of related genes. In this study, BMSCs, induced by myocardial lysate was used to observe the changes of cell morphology during differentiation. According to the changes of cell morphology during cardiomyogenesis, five detection points were selected and the expression of Nkx2.5 in the cells was detected by PCR. The purpose of this study was to explore the relationship between the morphological changes of cells and the expression of related genes in the process of differentiation. The results showed that during the process of differentiation of BMSCs into cardiomyocytes, the morphological changes of the cells showed obvious regularity: from a single nucleus, full cytoplasm and good refractive spindle, gradually evolved into short rod, formed protuberance and joined into reticular structure, and developed into a short rod, formed a protuberance and joined together into a reticular structure. The terminal phase of the cells was changed as "bamboo node-like", and finally the myotubule-like structure was formed. During the induction process, the cells did not express Nkx2.5, and the Nkx2.5 continued to express and gradually increased with the continuous changes of the cell morphology. These results suggest that the relative amount of Nkx2.5 expression may be related to the morphological changes of MSCs in the process of differentiation into cardiomyocytes.
【作者单位】： 广西医科大学组织学与胚胎学教研室;
【基金】：广西自然科学基金(2012GXNSFAA053126) 广西科技厅资助项目(桂科自0832157)、广西科技厅资助项目(桂科攻0592007-1G)、广西科技厅资助项目(桂科青0229009)共同资助
【分类号】：R329.2
本文编号：2471699
[Abstract]:During the differentiation of BMSCs into cardiomyocytes in vitro, there were obvious changes in morphology and expression of related genes. In this study, BMSCs, induced by myocardial lysate was used to observe the changes of cell morphology during differentiation. According to the changes of cell morphology during cardiomyogenesis, five detection points were selected and the expression of Nkx2.5 in the cells was detected by PCR. The purpose of this study was to explore the relationship between the morphological changes of cells and the expression of related genes in the process of differentiation. The results showed that during the process of differentiation of BMSCs into cardiomyocytes, the morphological changes of the cells showed obvious regularity: from a single nucleus, full cytoplasm and good refractive spindle, gradually evolved into short rod, formed protuberance and joined into reticular structure, and developed into a short rod, formed a protuberance and joined together into a reticular structure. The terminal phase of the cells was changed as "bamboo node-like", and finally the myotubule-like structure was formed. During the induction process, the cells did not express Nkx2.5, and the Nkx2.5 continued to express and gradually increased with the continuous changes of the cell morphology. These results suggest that the relative amount of Nkx2.5 expression may be related to the morphological changes of MSCs in the process of differentiation into cardiomyocytes.
【作者单位】： 广西医科大学组织学与胚胎学教研室;
【基金】：广西自然科学基金(2012GXNSFAA053126) 广西科技厅资助项目(桂科自0832157)、广西科技厅资助项目(桂科攻0592007-1G)、广西科技厅资助项目(桂科青0229009)共同资助
【分类号】：R329.2
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