SD大鼠肝脏枯否细胞分离方法改进研究
发布时间：2019-05-08 10:23
【摘要】：目的研究一种简单、高效、实用并且稳定的SD大鼠肝脏原代枯否细胞(KCs)的提取及培养方法。方法选择体质量200 g左右的健康SD雄性大鼠,采用0.05%IV型胶原酶在体原位灌注法结合剪碎法,37℃水浴振荡20 min,经30%和60%Percoll密度梯度离心后,再经40%Percoll离心及贴壁法纯化提取KCs。倒置显微镜下观察细胞形态,并用台盼蓝染色实验鉴定细胞活力,吞墨实验观察细胞吞噬功能及流式细胞术鉴定细胞纯度。结果每只大鼠平均肝脏KCs的获得量为(1.00±0.20)×107(n=20),细胞活力为(92.34±0.15)%,细胞纯度均在(93.56±0.24)%。结论改良的SD大鼠肝脏KCs提取方法操作简单,效果可靠,可为今后KCs的研究奠定基础。
[Abstract]:Aim to study a simple, efficient, practical and stable method for the extraction and culture of (KCs) from primary Kupffer cells of SD rat liver. Methods healthy male SD rats, weighing about 200g, were injected with 0.05% type IV collagenase in situ combined with shear method. After 30% and 60%Percoll density gradient centrifugation, 20 min, oscillated in water bath at 37 鈩，
本文编号：2471835
[Abstract]:Aim to study a simple, efficient, practical and stable method for the extraction and culture of (KCs) from primary Kupffer cells of SD rat liver. Methods healthy male SD rats, weighing about 200g, were injected with 0.05% type IV collagenase in situ combined with shear method. After 30% and 60%Percoll density gradient centrifugation, 20 min, oscillated in water bath at 37 鈩，
本文编号：2471835
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