组蛋白去乙酰化调控软骨细胞Ⅱ型胶原表达的机理研究
发布时间：2019-08-02 18:51
【摘要】：目的探讨通过TSA抑制组蛋白去乙酰化水平,研究组蛋白去乙酰化对软骨细胞表型相关基因的影响及其机制,从表观遗传学角度为维持软骨细胞表型提供新的思路。方法体外培养人关节软骨细胞,建立软骨细胞去分化模型,采用不同浓度TSA刺激,在不同时间点收集细胞,提取总RNA,利用qRT-PCR检测Wnt-5a、SOX-9、COL-Ⅱ、COL-Ⅰ的mRNA表达情况。利用免疫荧光及Westerblot进行COL-Ⅱ蛋白表达水平的检测。结果与对照组比较,TSA(0.25~1.0μmol/L)显著降低COL-ⅡmRNA表达水平及蛋白表达水平,升高Wnt-5a和SOX-9 mRNA表达水平;抑制Wnt-5a信号通路减弱TSA对COL-Ⅱ的抑制效应。结论 TSA通过激活Wnt-5a和SOX-9从而抑制COL-Ⅱ的表达,导致软骨细胞表型丧失。因此,组蛋白去乙酰化通过降低Wnt-5a和SOX-9,升高COL-Ⅱ的表达水平,发挥维持软骨细胞表型的作用。
[Abstract]:Objective to investigate the effect of TSA inhibition histone deacetylation on the phenotypic related genes of cartilage cells and its mechanism, and to provide a new idea for maintaining the phenotype of cartilage cells from the perspective of epigenetics. Methods Human articular cartilage cells were cultured in vitro and the model of cartilage dedifferentiation was established. The cells were collected at different time points stimulated by different concentrations of TSA. The total RNA, was extracted and the mRNA expression of Wnt-5a,SOX-9,COL- 鈪，
本文编号：2522302
[Abstract]:Objective to investigate the effect of TSA inhibition histone deacetylation on the phenotypic related genes of cartilage cells and its mechanism, and to provide a new idea for maintaining the phenotype of cartilage cells from the perspective of epigenetics. Methods Human articular cartilage cells were cultured in vitro and the model of cartilage dedifferentiation was established. The cells were collected at different time points stimulated by different concentrations of TSA. The total RNA, was extracted and the mRNA expression of Wnt-5a,SOX-9,COL- 鈪，
本文编号：2522302
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