电刺激点燃癫痫大鼠的皮层脑电及行为学研究

发布时间：2017-12-28 04:15

本文关键词：电刺激点燃癫痫大鼠的皮层脑电及行为学研究　出处：《中国比较医学杂志》2015年12期 　论文类型：期刊论文

【摘要】：目的应用Sprague-Dawley(SD)大鼠建立难治性癫痫杏仁核电刺激点燃癫痫模型,观察电刺激点燃模型的皮层脑电变化。方法用雄性SD大鼠,将双极电极立体定向植入大鼠右侧杏仁核,术后2周测定大鼠后放电阈值(after discharge threshold,ADT),用快速点燃方案建立杏仁核电刺激点燃癫痫模型。2周后再次点燃。应用皮层脑电图(electrocorticogram,ECoG)记录动物皮层脑电变化,应用视频监测仪记录大鼠的行为学改变。结果大鼠在测ADT过程中表现1~3级发作,ECoG显示异常放电;电刺激点燃时大鼠首先表现出1~3级发作,发作程度逐渐加剧直到出现4~5级发作,脑电图显示棘波、尖波、棘-慢波和尖-慢波等。统计学结果:首次点燃ADT:(82.33±21.29)μA,2周后ADT:(83.17±15.76)μA,P=0.923,差异无统计学意义;首次点燃电刺激点燃动物出现第一个4级发作所需要的电脉冲串的个数:(4.41±2.27),2周后:(5.58±3.96),P=0.231,差异无统计学意义。结论快速点燃癫痫动物模型是一个有效的癫痫模型,其稳定性在2周内持续存在。
[Abstract]:Objective to use Sprague-Dawley (SD) rats to establish epilepsy model of intractable epilepsy with amygdala nuclear stimulation, and to observe the changes of cortical electroencephalogram (EEG) in the electric stimulation kindling model. Methods male SD rats were implanted into the right amygdala by bipolar electrodes. The after discharge threshold (ADT) was measured 2 weeks after operation, and the amygdala kindled kindled epilepsy model was established by rapid kindling. Rekindle after 2 weeks. Electrocorticogram (ECoG) was used to record the changes of electroencephalogram (EEG) in the animal cortex, and the behavioral changes of rats were recorded by a video monitor. Results in the process of measuring ADT, rats showed 1~3 grade seizures, and ECoG showed abnormal discharge. When electrical stimulation ignited, the rats first showed 1~3 grade seizures, and the degree of exacerbation increased until 4~5 grade seizures appeared. EEG showed spike waves, sharp waves, spike slow waves, and sharp slow waves. Statistical results: first lit ADT: (82.33 + 21.29) A, ADT: after 2 weeks (83.17 + 15.76) A, P=0.923, the difference was not statistically significant; lit the first electrical kindling animal first 4 episodes required electric pulse number: (4.41 + 2.27) after 2 weeks.: (5.58 + 3.96), P=0.231, the difference was not statistically significant. Conclusion the fast kindling model of epileptic animal is an effective model of epilepsy, and its stability persists within 2 weeks.
【作者单位】：首都医科大学附属北京潞河医院;首都医科大学宣武医院;
【分类号】：R742.1;R-332
【正文快照】： 。癫痫动物模型的建立方法有多种,其中电刺激点燃模型与人类颞叶癫痫的发生、形成具有高度的相似性,并且具有精确的致痫灶,易于监测痫性发作,尤其适于难治性癫痫方面的研究[1-3]。电刺激点燃模型由Goddard首先引入医学领域,通过应用惊厥阈值下的电流反复刺激前脑的某一区域,使

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