改良线栓法大鼠脑缺血再灌注损伤模型的建立

发布时间：2018-01-03 20:01

本文关键词：改良线栓法大鼠脑缺血再灌注损伤模型的建立　出处：《中国医学创新》2015年03期 　论文类型：期刊论文

【摘要】：目的:通过改良线栓法建立稳定的Wistar大鼠大脑中动脉脑缺血/再灌注模型。方法:60只雄性Wistar大鼠,参考Longa法并适当改进建立大鼠大脑中动脉脑缺血/再灌注模型30只,对照组30只。实验组结扎右侧颈总动脉近心端,夹闭颈总动脉远心端,颈总动脉剪一小口,从颈总动脉经颈内动脉插入线栓,缺血1 h后,在麻醉状态下完全拔出线栓,并结扎颈内动脉远心端。对照组结扎右侧颈内动脉近心端及远心端,但不插入线栓。术后取脑组织行TTC染色,并进行神经功能评定。结果:经改良线栓法制作脑缺血再灌注损伤模型,成功率达82.75%,平均操作时间约(20.4±5.3)min,模型大鼠右侧眼裂变小,行走时向右侧转圈,模型大鼠神经功能评分均在3~4分,经TTC染色梗死侧大脑半球颜色苍白,脑梗死区范围一致,梗死体积约(34.62±5.32)%;对照组无任何临床症状,无神经功能缺失表现,经TTC染色无梗死灶。结论:通过改良,成功建立了Wistar大鼠大脑中动脉脑缺血/再灌注模型。
[Abstract]:Objective: to establish a stable cerebral ischemia / reperfusion model of middle cerebral artery in Wistar rats by modified thread embolization. Methods: 60 male Wistar rats were used. The model of cerebral ischemia / reperfusion of middle cerebral artery was established in 30 rats and 30 rats in control group. The proximal end of the right common carotid artery was ligated in the experimental group and the distal end of the common carotid artery was clamped. A small cut of the common carotid artery was cut and the thread embolus was inserted through the internal carotid artery from the common carotid artery. After 1 hour of ischemia, the thread plug was completely pulled out under anesthesia. The distal end of the internal carotid artery was ligated. In the control group, the proximal and distal ends of the right internal carotid artery were ligated, but no thread embolus was inserted. TTC staining was performed in the brain tissue after operation. Results: the model of cerebral ischemia-reperfusion injury was made by modified thread embolus method. The success rate was 82.75, and the average operating time was about 20.4 卤5.3 minutes. The right eye fissure of the model rats became smaller and the right side of the model rats walked around to the right. The neurological function scores of the model rats were all in the range of 3 ~ 4. The cerebral hemispheres of the infarcted side were pale and the infarct area was the same after TTC staining. The infarct volume was about 34.62 卤5.32. In the control group, there were no clinical symptoms, no neurological deficit and no infarct by TTC staining. Conclusion: the model of cerebral ischemia / reperfusion of middle cerebral artery in Wistar rats was successfully established by modified method.
【作者单位】：青海省人民医院;青海大学附属医院;海南医学院附属医院;北京市普仁医院;
【基金】：青海省科技计划项目(2012-Z-722)
【分类号】：R-332;R743
【正文快照】： Modified Establishment of Wistar Rats Ischemia-Reperfusion Injury Model/CHEN Xiao-juan,XIAOZong-yu,PAN Qi,et al.//Medical Innovation of China,2015,12(03):024-027缺血性脑血管病(Ischemic cerebral vascular disease,ICVD)是神经科的常见病、多发病,缺血后再灌注

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