脑源性神经营养因子在着床前小鼠胚胎中的表达及其对胚胎发育的影响

发布时间：2018-01-06 18:34

本文关键词：脑源性神经营养因子在着床前小鼠胚胎中的表达及其对胚胎发育的影响　出处：《生殖医学杂志》2015年01期 　论文类型：期刊论文

【摘要】：目的检测脑源性神经营养因子(BDNF)及其受体TrkB在植入前胚胎中的表达情况,探讨BDNF对小鼠早期胚胎发育和着床过程中的作用。方法收集小鼠受精后1~4d的胚胎,利用免疫组化和逆转录聚合酶链反应(RT-PCR)检测BDNF及其受体TrkB在各阶段胚胎中的表达情况。将2细胞阶段的胚胎放入添加不同浓度的BDNF的培养基中,添加或不添加TrkB受体阻断剂k252a/k252b,观察胚胎发育情况,并对形成的囊胚进行细胞计数。在注射人绒毛膜促性腺激素(HCG)后72h、76h、84h和88h分别腹腔注射10μg K252a/k252b,收集胚胎,计算扩张期囊胚数和囊胚细胞数。结果 BDNF及TrkB均表达于小鼠植入前各阶段的胚胎中,在培养基中添加BDNF后能够促进小鼠胚胎的体外发育,能够提高囊胚形成率和囊胚孵化率,并能够提高滋养层细胞数,此作用能够通过TrkB受体阻断剂k252a而阻断。体内研究进一步证实,k252a处理后能够抑制囊胚细胞数而明显抑制早期胚胎发育。结论 BDNF/TrkB信号系统可能通过某种自分泌或旁分泌的作用方式促进植入前胚胎的发育,同时也可能参与胚胎的着床过程。
[Abstract]:Objective to investigate the expression of brain-derived neurotrophic factor (BDNF) and its receptor TrkB in preimplantation embryos. To investigate the effect of BDNF on the development and implantation of mouse early embryos. Using immunohistochemistry and reverse transcriptase polymerase chain reaction (RT-PCR). The expression of BDNF and its receptor TrkB in different stages of embryo was detected. The 2-cell embryos were added to the medium with different concentrations of BDNF. TrkB receptor antagonist k252a / k252b was added or not, and embryo development was observed. The blastocysts were counted, and 10 渭 g K252a / k252b was injected intraperitoneally at 72 h, 76 h, 84 h and 88 h after injection of HCG, human chorionic gonadotropin (HCG). The number of blastocysts and the number of blastocyst cells were calculated. Results BDNF and TrkB were expressed in mouse embryos before implantation. The addition of BDNF to the medium could promote the development of mouse embryos in vitro, increase the blastocyst formation rate and blastocyst hatching rate, and increase the number of trophoblast cells. This effect can be blocked by TrkB receptor blocker k252a. Further confirmed in vivo. K252a treatment could inhibit the number of blastocyst cells and inhibit the development of early embryos. BDNF/TrkB signaling system may promote the development of preimplantation embryos through some autocrine or paracrine mechanism. It may also be involved in the implantation of embryos.
【作者单位】：中国医学科学院北京协和医学院北京协和医院妇产科生殖中心;
【分类号】：R321
【正文快照】： Results:Both BDNF and TrkB were expressed in all stage of preimplantation embryos.Treatmentwith BDNF promoted the development of two-cell-stage embryos into blastocysts in vitro,increased theblastocyst formation rate and hatching rate,and the total numbe

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