人和大鼠脊髓显微结构连续切片图像的计算机三维重建研究

发布时间：2018-01-18 04:21

本文关键词：人和大鼠脊髓显微结构连续切片图像的计算机三维重建研究　出处：《南通大学》2008年硕士论文　论文类型：学位论文

【摘要】： 目的:利用人和大鼠脊髓组织连续切片,结合三维重建工具软件对人、正常大鼠脊髓显微结构进行三维重建,并对重建的结构进行观察和分析。方法:(1)对人腰髓节段的连续冰冻切片直接贴片,进行Luxol Fast Blue (LFB)染色,采集切片图像并进行图像拼接,Photoshop7.0软件对图像进行“图像灰度化”、“反相”、“对比度调整”、“图像缩小”等后处理,3D-DOCTOR4.0软件进行表面重建和体重建。(2)制作正常SD大鼠颈髓节段包埋蜡块,于颈髓标本四周进行外定位标记,Leica石蜡切片机进行连续横断切片,每切三张,用CANON数码相机对蜡块中的颈髓标本及其外定位标记进行拍照,获得连续切片图像。所得图像进行排序,裁切,转换为灰度图像和背景不均匀校正后,利用3D-DOCTOR软件对每张图像上的颈髓,颈髓灰质,定位孔进行边缘提取,利用定位孔对连续图像进行自动配准,三维表面重建后在PC机上进行任意角度观察,切割和测量。(3)制作正常成年SD大鼠脊髓连续横断切片、贴片后,进行LFB染色、拍片,获得大鼠脊髓皮质脊髓束连续切片图像。在Photoshop软件环境下完成切片图像的配准、分割、灰度化。利用3D-DOCTOR软件进行阈值分割并对大鼠脊髓、脊髓灰质、双侧皮质脊髓束结构进行三维重建,在PC机上对重建结构进行任意角度的观察,切割和测量。结果:(1)利用3D-DOCTOR软件重建出腰髓节段,可以自由观察腰髓的结构,并进行测量。另外还可以选择任意切面切割腰髓节段,观察并测量剩余部分的结构。(2)利用3D-DOCTOR软件重建出大鼠颈髓节段,可以自由观察颈髓的结构,并进行测量。另外还可以选择任意切面切割颈髓节段观察并测量剩余部分的结构。(3)利用大鼠脊髓连续切片重建出C1-S4节段大鼠脊髓,在PC机上对其进行任意角度的观察和切割观察,大鼠皮质脊髓束于大鼠C1-S4节段走行于大鼠脊髓后索内,紧贴灰质中间带后方,呈逐渐变细的类圆柱状形态。结论:(1)利用人腰髓连续切片可以进行人体脊髓的三维重建,并进一步对脊髓内部结构进行三维可视化研究。(2)利用石蜡包埋和“硬”定位技术可以获得颈髓节段连续横断切面图像,进行三维重建并对重建结构进行自由观察和测量。(3)利用大鼠脊髓的连续LFB染色切片,结合计算机三维重建技术能够进行大鼠皮质脊髓束的三维重建,并提供测量数据。
[Abstract]:Aim: to reconstruct the microstructures of human and normal rat spinal cord by serial sections of human and rat spinal cord, and to observe and analyze the reconstructed structure. Methods Luxol Fast Blue (LFB) staining was performed on the continuous frozen slices of human lumbar spinal cord segment. The slice images were collected and stitched. Photoshop7.0 software carries on "image grayscale", "inverse phase", "contrast adjustment", "image shrinks" and so on post-processing. 3D-DOCTOR4.0 software was used for surface reconstruction and body weight building. (2) normal Sprague-Dawley rat cervical spinal cord segment embedded wax mass was made, and the external localization labeling was carried out around the cervical spinal cord specimen. The Leica paraffin slicing machine was used to slice the cervical spinal cord and its external localization markers. The cervical spinal cord specimens in the wax block were photographed with CANON digital camera. The serial slice images were obtained. The images were sorted, cut, converted to gray images and corrected for background inhomogeneity, and the gray matter of cervical spinal cord and cervical spinal cord on each image was analyzed by 3D-DOCTOR software. The edge of the location hole is extracted, the continuous image is automatically registered by the location hole, the 3D surface is reconstructed and the angle is observed at any angle on the PC. The spinal cord of adult Sprague-Dawley rats was cut and measured. The spinal cord of normal adult SD rats was cut and measured. The slices were stained with LFB and photographed. The serial slice images of corticospinal tract of rat were obtained. The registration and segmentation of the slice images were completed under the environment of Photoshop software. Using 3D-DOCTOR software for threshold segmentation and 3D reconstruction of spinal cord, gray matter and bilateral cortical spinal tract structure in rats. Observe, cut and measure the reconstructed structure at any angle on PC. Results 1) using 3D-DOCTOR software to reconstruct the lumbar spinal cord segment, the structure of the lumbar spinal cord can be observed and measured freely, and the lumbar spinal cord segment can be cut on any section. The structure of the remaining part was observed and measured.) the cervical spinal cord segment was reconstructed by 3D-DOCTOR software and the structure of the cervical spinal cord was observed freely. In addition, we can choose to cut the cervical spinal cord segment on any section and measure the structure of the remaining part.) the C1-S4 segment spinal cord can be reconstructed by continuous slice of rat spinal cord. The cortical spinal tract of the rat was observed at any angle and incised on the PC computer. The cortical spinal tract of the rat was located in the back of the spinal cord of the rat at the segment of C1-S4, which was close to the posterior middle band of gray matter. A cylindrical shape that gradually becomes thinner. Conclusion 1) the three-dimensional reconstruction of human spinal cord can be carried out by continuous section of human lumbar spinal cord. Further 3D visualization of the internal structure of the spinal cord. 2) using paraffin embedding and "hard" localization technology, we can obtain continuous cross-sectional images of cervical spinal cord segment. Three-dimensional reconstruction and free observation and measurement of the reconstructed structure.) Three-dimensional reconstruction of rat cortical spinal tract can be carried out by using continuous LFB staining sections of rat spinal cord and computer three-dimensional reconstruction technique. And provide measurement data.
【学位授予单位】：南通大学
【学位级别】：硕士
【学位授予年份】：2008
【分类号】：R329;R319

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