丹参对AD模型小鼠学习记忆及脑内和血清内乙酰胆碱酯酶含量的影响

发布时间：2018-01-19 15:41

  本文关键词： 阿尔兹海默病 丹参 三氯化铝 乙酰胆碱酯酶　出处：《实验动物科学》2014年04期 　论文类型：期刊论文

【摘要】：目的观察丹参对三氯化铝(AlCl3)所致的阿尔兹海默病(AD)模型小鼠学习记忆能力的影响及其脑组织和血清中乙酰胆碱酯酶(AchE)含量的变化。方法 AD模型组:给小鼠每日按400 mg/kg灌胃4%的AlCl3溶液,连续灌胃60 d。正常对照组:每日灌胃与AlCl3等同剂量的生理盐水。丹参治疗组:每日灌胃AlCl3+丹参溶液1 mL。丹参对照组:每日只灌胃丹参溶液1 mL。实验周期为60 d。给药结束后进行Morris水迷宫训练,24 h后进行学习记忆功能测试和乙酰胆碱酯酶含量的测定。结果丹参治疗组、丹参对照组与正常对照组比较,小鼠的逃避潜伏期以及其脑组织和血清中的乙酰胆碱酯酶含量无明显变化,差异无统计学意义(P0.05)。与模型组小鼠比较,丹参治疗组小鼠的逃避潜伏期明显缩短(P0.05),并且其脑组织和血清中的乙酰胆碱酯酶含量比模型组明显降低(P0.05)。结论丹参能有效降低AlCl3致AD模型小鼠脑内及血清中胆碱酯酶活性,明显改善AD模型小鼠的学习记忆功能,这可能是丹参可防治阿尔兹海默病的机制之一。
[Abstract]:Objective to observe the effect of Salvia miltiorrhiza on learning and memory ability and acetylcholinesterase (ache) in brain tissue and serum of mice with Alzheimer's disease induced by AlCl3). Methods in AD model group, the mice were given 4% AlCl3 solution at 400 mg/kg per day. For 60 days, the normal control group was given the same dose of normal saline as AlCl3 daily. The salvia miltiorrhiza treated group was given AlCl3 Salvia miltiorrhiza solution 1 mL daily. The control group was treated with Salvia miltiorrhiza. Only 1 mL of Salvia miltiorrhiza solution was administered daily for 60 days. Morris water maze training was performed after administration. After 24 hours, the function of learning and memory and the content of acetylcholinesterase were measured. Results compared with the normal control group, the salvia miltiorrhiza treatment group and the salvia miltiorrhiza control group. The escape latency and the content of acetylcholinesterase in brain tissue and serum of mice showed no significant change, but the difference was not statistically significant (P 0.05). The escape latency of salvia miltiorrhiza treated mice was shortened significantly (P0.05). The content of acetylcholinesterase in brain tissue and serum was significantly lower than that in model group. Conclusion Salvia miltiorrhiza can effectively reduce the activity of cholinesterase in brain and serum of AD model mice induced by AlCl3. It may be one of the mechanisms that Salvia miltiorrhiza can prevent and treat Alzheimer's disease.
【作者单位】： 长沙医学院基础医学院;
【基金】：长沙医学院大学生研究性学习和创新性实验资助项目(长医教[2012]21号) 湖南省卫生厅一般项目(No.B2013-059) 湖南省教育厅一般项目(No.12C0509)
【分类号】：R749.16
【正文快照】： 阿尔兹海默病(Alzheimers disease,AD)是一种与年龄相关的中枢神经系统退行性病变[1],发病率较高,已成为现代社会严重威胁老年人生命的疾病之一,其死亡率仅次于心血管疾病、癌症及中风而位居第四[2]。铝是一种慢性神经毒物,可在脑组织中蓄积,干扰脑细胞活动,破坏神经元结构,导，

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