IL-27在小鼠CLP-急性肺损伤模型中的作用

发布时间：2018-01-20 10:24

本文关键词： 急性肺损伤 IL- CLP 炎症细胞因子　出处：《免疫学杂志》2015年06期 　论文类型：期刊论文

【摘要】：目的研究细胞因子IL-27在急性肺损伤中发挥的作用和相关机制。方法采用盲肠结扎穿孔术(CLP)建立小鼠急性肺损伤模型。ELISA和荧光实时定量PCR法检测CLP-急性肺损伤小鼠模型的肺组织、支气管灌洗液、外周血单个核细胞及血清中IL-27及其亚单位的表达。同时,小鼠行CLP术前吸入给药IL-27中和抗体,经组织学检测小鼠肺组织炎症水平的改变,并检测支气管灌洗液中IL-6、CXCL10、IFN-γ的水平和小鼠存活率。结果 CLP-小鼠的肺组织和外周单个核细胞中IL-27的2个亚单位(EBI3和p28)均增加,支气管灌洗液和血清中的IL-27水平也较对照组显著增加(P0.01)。抗IL-27多克隆抗体的使用降低了CLP-急性肺损伤小鼠肺组织的炎症水平,支气管灌洗液中IL-6、CXCL10、IFN-γ水平下降(P0.05),同时提高了小鼠存活率。结论IL-27在急性肺损伤中发挥重要作用,抑制IL-27的表达可能成为预防和治疗急性肺损伤的新途径。
[Abstract]:Objective to study the role and mechanism of cytokine IL-27 in acute lung injury. An acute lung injury model was established in mice. Elisa and fluorescence real-time quantitative PCR were used to detect the lung tissue of CLP- acute lung injury mice. The expression of IL-27 and its subunits in peripheral blood mononuclear cells and serum in bronchial lavage fluid. At the same time, IL-27 neutralizing antibody was inhaled before CLP in mice. The changes of lung inflammation and IL-6 CXCL10 in bronchial lavage fluid were detected by histology. Results the levels of IFN- 纬 and the survival rate of mice were increased in both lung tissue and peripheral mononuclear cells (EBI3 and p28) of IL-27. The level of IL-27 in bronchial lavage fluid and serum was also significantly higher than that in control group (P 0.01). The use of anti IL-27 polyclonal antibody decreased the inflammatory level of lung tissue in CLP- acute lung injury mice. The level of IL-6 CXCL10 IFN- 纬 in broncholavage fluid decreased P0.05 and increased the survival rate of mice. Conclusion IL-27 plays an important role in acute lung injury. Inhibiting the expression of IL-27 may be a new way to prevent and treat acute lung injury.
【作者单位】：重庆医科大学附属第一医院输血科;重庆医科大学附属第一医院检验科;
【基金】：国家重点专科建设项目(财社[2010]305号)
【分类号】：R563;R-332
【正文快照】： 急性肺损伤可以诱发多种严重并发症,如败血症、肺炎、急性呼吸窘迫综合征、缺血/再灌注和胰腺炎等[1-2]。它的发病机制是全身炎症反应的失控,包括微循环损伤、肺血管通透性增加、肺间质以及肺泡水肿和低氧性呼吸衰竭。研究发现[3-5],急性肺损伤时,白细胞和肺泡细胞会释放大量

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