BDE-209对小鼠卵母细胞体外成熟和孤雌激活后孤雌胚发育的影响

发布时间：2018-02-25 00:12

  本文关键词： 十溴联苯醚 小鼠 卵母细胞 体外成熟 体外培养 孤雌生殖　出处：《暨南大学》2009年硕士论文　论文类型：学位论文

【摘要】： 目的: 研究十溴联苯醚(brominated diphenyl ethers-209,BDE-209)对小鼠卵母细胞体外成熟过程中生发泡破裂率、第一极体释放率和卵母细胞存活率的影响,并对培养至成熟卵母细胞行体外孤雌激活处理,研究孤雌激活后BDE-209对胚胎发育的影响。 方法: 通过对性成熟未孕健康母鼠进行PMSG+HCG超排卵处理,取得成熟卵母细胞。将0μg/ml BDE-209作为对照组A,10、20、40μg/ml作为实验组B、C、D,用含A、B、C、D不同浓度BDE-209的DMEM(低糖)培养液行体外培养,观察各组在8小时和16小时的第一极体释放率和卵母细胞存活率,并比较其差异。用PMSG促排卵处理从性未成熟小鼠体内获得未成熟卵母细胞行体外培养,于12小时观察卵母细胞生发泡破裂率,于24小时观察第一极体释放率。再将MⅡ期卵母细胞行乙醇孤雌激活处理,比较各组孤雌激活率是否有差异,将孤雌激活后的卵母细胞置于含不同浓度BDE-209的CZB培养液中行胚胎培养,于培养的24小时观察2-细胞形成率,于48小时观察4-细胞形成率,并比较其组间差异。 结果: 对成熟卵母细胞行体外培养,分别比较A、B、C、D各组8小时和16小时第一极体释放率,各组差别无统计学意义(P＞0.05)。于8小时和16小时两时段分别比较四组间其第一极体释放率差别均无统计学意义(P＞0.05);分别比较A、B、C、D各组8小时和16小时的卵母细胞存活率,差别无统计学意义(P＞0.05)。B、C、D各组16小时组比8小时组卵母细胞存活率均有所降低,P＜0.05,差别统计学意义。于8小时,16小时时段分别比较四组间卵母细胞存活率,P＞0.05,差别无统计学意义。 对未成熟卵母细胞行体外培养,比较各组12小时生发泡破裂率,P＞0.05,差别无统计学意义。比较24小时各组第一极体释放率,实验组比对照组均有明显降低,P＜0.05,差别有统计学意义。将各组培养所得成熟卵母细胞行孤雌激活处理,P＞0.05,各组的激活率无明显差异。 将孤雌激活后的卵母细胞行体外胚胎培养,于24小时观察2-细胞形成率,48小时观察4-细胞形成率,实验组较对照组有所降低,P＜0.05,差别有统计学意义。 结论: BDE-209对成熟卵母细胞的培养过程中,不抑制小鼠卵母细胞第一极体释放率,但降低卵母细胞的存活率。对未成熟卵母细胞的体外培养过程中,其不抑制生发泡破裂,但是使得第一极体释放率降低。对培养成熟的卵母细胞行孤雌激活处理,各组间激活率无显著性差异,但其对孤雌激活后胚胎发育有明显的抑制作用。
[Abstract]:Objective:. To study the effects of decabrominated diphenyl ethers-209 (BDE-209) on blastocyst rupture rate, first polar body release rate and oocyte survival rate during mouse oocytes maturation in vitro, and parthenogenetic activation of cultured mature oocytes in vitro. To study the effect of parthenogenetic activated BDE-209 on embryonic development. Methods:. The mature oocytes were obtained by superovulation of PMSG HCG on unpregnant and sexual mature healthy mice. 0 渭 g / ml BDE-209 was used as the control group (AH1010, 2040 渭 g / ml) as the experimental group, and DMEM (low glucose) culture medium containing different concentrations of BDE-209 was cultured in vitro. The first polar body release rate and oocyte survival rate at 8 and 16 hours in each group were observed and the difference was compared. Immature oocytes were obtained from immature mice by PMSG stimulation in vitro. The blastocyst rupture rate of oocytes was observed at 12 hours and the release rate of the first polar body was observed at 24 hours. Then the M 鈪，

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