年轻骨髓间充质干细胞可通过细胞融合改善年老骨髓间充质干细胞功能

发布时间：2018-02-25 02:24

本文关键词： 骨髓间充质干细胞年龄因素脂肪细胞成骨细胞细胞融合　出处：《中国病理生理杂志》2010年05期 　论文类型：期刊论文

【摘要】：目的:诱导年轻与年老小鼠骨髓间充质干细胞(BMSCs)融合,探讨融合后年轻BMSCs对年老BM-SCs功能的影响。方法:年轻(2-3月龄)及年老(18-24月龄)C57BL/6小鼠BMSCs在红色荧光染料PKH26标记后分别与年轻及年老绿色荧光蛋白转基因C57BL/6小鼠BMSCs通过聚乙烯二醇(PEG1500)诱导,建立细胞融合模型。通过流式细胞仪鉴定细胞融合率和细胞表面特异性抗原的表达。免疫荧光显微镜观察融合细胞形态及细胞核特性。通过细胞计数比较5组细胞(年轻组Y,年老组O,年轻融合组Y-Y,年轻年老融合组Y-O,年老融合组O-O)在2d、4d、6d、8d的细胞增殖能力。诱导细胞向成骨细胞及脂肪细胞分化,比较5组细胞的分化能力。结果:通过PEG诱导,可获得30.45%±4.13%的融合细胞,3组不同年龄融合组的细胞融合率无显著差异。融和细胞表达间充质干细胞表面特异性抗原CD44、Sca-1,而不表达造血干细胞表面特异性抗原CD34、CD117、CD31、CD45。在2d、4d、6d、8d,Y-O组细胞数量增加百分率比O-O组均显著增高。代表成骨细胞分化能力强弱的茜素红染色阳性率和代表脂肪细胞分化能力强弱的油红O染色阳性率,Y-O组比O-O组均显著增高,具体数值分别为:(25.46%±1.52%)vs(13.85%±1.69%),P0.01;(12.99%±2.61%)vs(6.03%±1.71%),P0.05。结论:年轻BMSCs可通过细胞融合改善年老BMSCs增殖及分化功能。
[Abstract]:Objective: to induce the fusion of bone marrow mesenchymal stem cells (BMSCs) in young and aged mice. To investigate the effect of young BMSCs on the function of aged BM-SCs after fusion. Methods: the BMSCs of young and aged C57BL / 6 mice were labeled with red fluorescent dye PKH26 and BMSCs of young and aged C57BL / 6 mice were treated with green fluorescent protein transgenic C57BL / 6 mice. Induced by PEG1500). Cell fusion model was established. Cell fusion rate and cell surface specific antigen expression were identified by flow cytometry. Morphology and nuclear characteristics of fusion cells were observed by immunofluorescence microscope. 5 groups of cells were compared by cell count. The proliferation ability of cells (young group, old group, young fusion group, young fusion group, young and old fusion group, young fusion group, old fusion group, O-O group) was induced to differentiate into osteoblasts and adipocytes at 2 d, 4 d, 6 d and 6 d, respectively. Results: the differentiation ability of 5 groups of cells was induced by PEG. There was no significant difference in cell fusion rate among the fusion cells of 30.45% 卤4.13% and different age groups. The fusion cells expressed mesenchymal stem cell surface specific antigen CD44-Sca-1, but did not express hematopoietic stem cell surface specific antigen CD34, CD117, CD31 and CD45. The fusion cells expressed the surface specific antigen of mesenchymal stem cells for 6 days and 8 days after 2 days. The positive rate of alizarin red staining representing the differentiation ability of osteoblasts and the positive rate of oil red O staining representing the differentiation ability of adipocytes were significantly higher in Y-O group than in O-O group. The specific values were 25.46% 卤1.52% + 13.85% 卤1.69% 卤1.69% and 12.99% 卤2.61% 卤6.03% 卤1.71% respectively. Conclusion: young BMSCs can improve the proliferation and differentiation of aged BMSCs through cell fusion.
【作者单位】：广州医学院第二附属医院心内科广州心血管疾病研究所;
【基金】：广东省科技计划资助项目(No.2006B50107005) 广州市教育系统首批建设创新学术团队资助项目(No.穗教科[2009]11号)
【分类号】：R329

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