不同浓度解脲支原体血清型3在小鼠模型中的致病性研究

发布时间：2018-03-21 17:47

本文选题：解脲支原体　切入点：血清型　出处：《中南大学》2008年硕士论文　论文类型：学位论文

【摘要】： 目的:探讨不同浓度解脲支原体血清型3(Uu3)在小鼠模型中的致病性。方法:将156只雌激素预处理过的昆明小鼠随机分为四组(其中A、B、C三个实验组每组48只,D组12只),分别阴道接种三种浓度Uu3及无菌培养液(A组接种浓度为10~7 copy/g,B组为10~6 copy/g,C组为10~5 copy/g,D组为对照组接种无菌培养液)。接种后第1、3、7、14、21、35天随机处死每实验组各8只及对照组2只小鼠,取宫颈管分泌物行Uu3的荧光定量聚合酶链反应(FQ-PCR)检测;取宫颈、子宫内膜及输卵管行HE染色光镜观察,取宫颈管粘膜扫描电镜观察。结果:(1)阳性率:3个实验组宫颈管分泌物中Uu3总阳性率分别为:62.5%(30/48),50.0%(24/4,),16.7%(8/48),差异有统计学意义(P=0.000)。随着接种后时间的延长,阳性率呈下降趋势,其中接种后第一天达高峰,A组、B组、C组的阳性率分别为:100.0%(8/8),87.5%(7/8),37.5%(3/8),至第35天均无检出。对照组均无Uu3检出。(2)定量值:3个实验组在接种后每一时间点(除第21天),宫颈管Uu3FQ-PCR定量值差异有统计学意义(P＜0.05),接种浓度越高,Uu3定量值越大。且定量值随接种后时间的延长逐渐下降,其中第1天达高峰,A组、B组、C组的定量值均数分别为:1.70×10~7 copy/g,3.75×10~6 copy/g,1.45×10~5 copy/g。(3)致病率:接种后第7～35天,A组、B组、C组的总致病率分别为:56.3%(18/32),43.8%(14/32),6.3%(2/32),差异有统计学意义(P=0.000);每一时间点,三个实验组的致病率亦不同,接种浓度越高,致病率越高,且A组与B组致病率明显高于C组。对照组无致病。结论:(1)在小鼠模型中,Uu3在宫颈管的阳性检出率及定量值、致病率随接种浓度的增高而增高。 (2)在小鼠模型中,生殖道局部不同浓度的Uu3,其致病性不同。当Uu3浓度≥10~6 copy/g时,其致病性明显增强。
[Abstract]:Objective: to study the pathogenicity of Ureaplasma Urealyticum serotype 3 UU 3 in mouse model. Methods: 156 Kunming mice pretreated with estrogen were randomly divided into four groups (group A, n = 48) and group D (n = 12, n = 48). Vaginal inoculation of three concentrations of Uu3 and aseptic culture medium (group A, n = 10) was carried out at a concentration of 10 ~ 7 copy / g ~ (-1). After inoculating aseptic medium in the control group for 10 ~ 6 copy / g / g C group, 8 mice in each group and 2 mice in the control group were killed at random on the 35th day after inoculation. The secretion of cervical canal was detected by fluorescence quantitative polymerase chain reaction (FQ-PCR) of Uu3, the cervix, endometrium and fallopian tube were observed by HE staining, and the mucous membrane of cervix uteri was observed by scanning electron microscope. Results: the total positive rate of Uu3 in cervical canal secretions of the three experimental groups was: 30 / 48 / 48 / 50. 0. 0 / 48, respectively. The difference was statistically significant (P < 0. 000). With the extension of the time after inoculation, the positive rate showed a downward trend. On the first day after inoculation, the positive rates of group B and C were: 1: 100.0 and 87.5g / 87.5g / 87.5a, respectively, and no positive rate was detected at 37.5% on the 35th day after inoculation. In the control group, there was no Uu3 detection. 2) quantitative value: three experimental groups were tested at each time point after inoculation (except 21 days after inoculation, Uu3FQ-PCR in the cervix canal was detected at each time point). The difference of quantitative value was significant (P < 0.05). The higher the inoculation concentration was, the greater the quantitative value of UU3 was, and the quantitative value decreased gradually with the extension of time after inoculation. On the first day of the inoculation, the total pathogenicity of group C was: 1.70 脳 10 ~ 7 copyg 3.75 脳 10 ~ (6) / g ~ (1.45 脳 10 ~ (5) copy 路g ~ (3)): the total pathogenicity of group C in group A was 56.332% ~ 1832% on day 735 after inoculation, and the difference was statistically significant (P = 0.000); at each point of time, there was a significant difference in the total pathogenicity of group C and group B (P = 0.000, P = 0.000, P = 0.000, P = 0.000, P = 0.000, P = 0.000, P = 0.000, P = 0.000, P = 0.000, P = 0.000, P = 0.000, P = 0.000, P = 0.000, P = 0.000, P = 0.000). The pathogenicity of the three experimental groups was also different. The higher the inoculation concentration, the higher the pathogenicity. The pathogenicity of group A and group B was significantly higher than that of group C. the control group had no pathogenicity. Conclusion the positive detection rate and quantitative value of UU3 in cervical canal in mouse model increased with the increase of inoculation concentration. (2) in mouse model, the pathogenicity of UU3 in different concentrations of reproductive tract was different. When the concentration of Uu3 was more than 106 copy/g, the pathogenicity of UU3 was obviously enhanced.
【学位授予单位】：中南大学
【学位级别】：硕士
【学位授予年份】：2008
【分类号】：R375

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