应用Label-free技术研究人牙囊细胞和牙周膜成纤维细胞的差异蛋白质

发布时间：2018-04-12 18:10

本文选题：牙囊细胞(h + DFCs)　；参考：《牙体牙髓牙周病学杂志》2015年05期

【摘要】：目的:应用Label-free蛋白定量技术研究牙囊细胞(h DFCs)和牙周膜成纤维细胞(h PDLFs)差异表达蛋白质,为发现h DFCs向h PDLFs转化过程中的重要蛋白质提供参考。方法:应用Label-free蛋白定量技术,结合ultramate 3000 nano-UPLC软件,对h DFCs和h PDLFs总蛋白提取液进行蛋白定性定量检测。然后分别对蛋白质的鉴定结果行重复性、关联性、蛋白相对含量和差异表达蛋白等进行分析;对总体蛋白质和差异表达蛋白质行生物信息学GO或KEGG分析。结果:h DFCs和h PDLFs蛋白相对含量及总体蛋白GO分析结果相似。定量分析显示,当h DFCs分化形成h PDLFs后,有22个差异表达蛋白质,其中下调蛋白15个(P0.05,FC2),上调蛋白7个(P0.05,FC2)。结论:h DFCs和h PDLFs蛋白质表达谱相似;22个差异表达蛋白质为研究h DFCs向h PDLFs的转化机制提供了方向。
[Abstract]:Objective: to study the differential expression of Label-free protein between human dental follicle cells and periodontal ligament fibroblasts by using Label-free protein quantitative technique, and to provide a reference for the discovery of important proteins in the transformation of h DFCs to h PDLFs.Methods: using Label-free protein quantitative technique and ultramate 3000 nano-UPLC software, the protein of h DFCs and h PDLFs total protein extract were detected qualitatively and quantitatively.Then the reproducibility, correlation, relative protein content and differentially expressed protein were analyzed, and the total protein and differential expressed protein were analyzed by bioinformatics go or KEGG.Results the relative contents of DFCs and h PDLFs protein were similar to those of total protein by go analysis.Quantitative analysis showed that there were 22 differentially expressed proteins when h DFCs differentiated into h PDLFs, including 15 down-regulated proteins (P0.05FC2) and 7 up-regulated proteins (P0.05FC2P).Conclusion the protein expression profiles of h DFCs and h PDLFs are similar, and 22 differentially expressed proteins provide a direction for studying the transformation mechanism of h DFCs to h PDLFs.
【作者单位】：军事口腔医学国家重点实验室陕西省口腔医学重点实验室第四军医大学口腔医院正畸科;解放军第
【基金】：国家自然科学基金面上项目(81271176)
【分类号】：R329.2

【共引文献】