库仑阵列电化学-高效液相色谱法同时检测大鼠不同脑区中的8种单胺类神经递质

发布时间：2018-05-10 18:23

本文选题：单胺类神经递质 + 库仑阵列电化学检测　；参考：《广东医学院学报》2015年06期

【摘要】：目的建立库仑阵列电化学-高效液相色谱法同时测定大鼠海马、皮质、下丘脑中8种单胺类神经递质的分析方法。方法采用库仑阵列电化学高效液相色谱仪检测,以内标法测定大鼠不同脑区中单胺类神经递质及其相关代谢产物的含量。结果 8种单胺类神经递质在本实验条件下线性关系良好,最低检测限在0.245~0.330μg/L之间,平均回收率均在87.0%~98.3%之间,大鼠不同脑区中8种单胺类神经递质的含量均在28.2~2472.8 ng/g之间。结论该方法具有操作简便、快速、准确、灵敏、分离度好、仪器稳定等优点,可以用于大鼠不同脑区8种单胺类神经递质的同时检测与分析。
[Abstract]:Objective to establish a method for simultaneous determination of 8 monoamine neurotransmitters in rat hippocampus, cortex and hypothalamus by Coulomb array electrochemical and high performance liquid chromatography. Methods the contents of monoamine neurotransmitters and related metabolites in different brain regions of rats were determined by Coulomb array electrochemical high performance liquid chromatography and internal standard method. Results the linear relationship of 8 monoamine neurotransmitters was good in this experiment. The lowest detection limit was between 0.245 渭 g / L and 0.330 渭 g / L, and the average recovery was between 87.0% and 98.3%, and the contents of 8 monoamine neurotransmitters in different brain regions of rats were between 28.2n 2472.8 ng/g. Conclusion this method has the advantages of simple, rapid, accurate, sensitive, good separation and stable instrument. It can be used for the simultaneous detection and analysis of 8 monoamine neurotransmitters in different brain regions of rats.
【作者单位】：广东医学院第二临床医学院;广州中医药大学中药学院;
【基金】：国家自然科学基金项目(No.81403085) 东莞市医疗卫生科技计划一般项目(No.2014105101295) 广东医学院科研基金博士学位人员科研启动项目(No.B2013007)
【分类号】：R-33

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