成骨细胞中雌激素受体β介导雌激素对IL-6和TGF-β的调控作用

发布时间：2018-05-10 19:19

本文选题：雌激素 + IL-　；参考：《中国矫形外科杂志》2015年09期

【摘要】：[目的]利用小鼠胚胎成骨细胞前体细胞MC3T3-E1为细胞模型,研究雌激素与IL-6和TGF-β三者在成骨细胞分化中的具体作用机制,进一步在细胞水平探讨雌激素受体ERβ在雌激素功能中的作用。[方法]17β-雌二醇处理MC3T3-E1细胞后检测碱性磷酸酶活性、骨桥蛋白、骨保护素、骨钙素等成骨细胞相关因子的表达。同时17β-雌二醇处理后检测细胞因子IL-6和TGF-β的表达是否改变。然后分别用IL-6和TGF-β单独处理后检测MC3T3-E1细胞成骨分化相关因子和破骨细胞分化因子的表达。进一步利用siRNA干扰ERβ的表达,检测在17β-雌二醇诱导下IL-6和TGF-β表达的改变。[结果]雌激素能够诱导MC3T3-E1细胞碱性磷酸酶活性、骨桥蛋白、骨保护素、骨钙素的生成。雌激素处理后细胞因子IL-6的生成受到抑制,而TGF-β的表达上调。干扰了ERβ后,17β-雌二醇处理MC3T3-E1细胞后白细胞介素6及TGF-βmRNA的表达无明显改变。[结论]雌激素通过ERβ,调控IL-6和TGF-β的表达,进而调节成骨细胞的功能和骨的形成。
[Abstract]:[objective] to study the mechanism of estrogen, IL-6 and TGF- 尾 in osteoblast differentiation by using mouse embryonic osteoblast precursor cell (MC3T3-E1) as cell model. To further explore the role of estrogen receptor ER 尾 in estrogen function at the cell level. [methods] the activity of alkaline phosphatase, osteopontin, osteocalcin and osteocalcin were detected in MC3T3-E1 cells treated with 17 尾 -estradiol. After 17 尾 -estradiol treatment, the expression of cytokines IL-6 and TGF- 尾 was detected. Then the expression of osteogenic differentiation related factors and osteoclast differentiation factors in MC3T3-E1 cells were detected by IL-6 and TGF- 尾 alone. Furthermore, the expression of ER 尾 was interfered by siRNA, and the changes of IL-6 and TGF- 尾 expression induced by 17 尾 -estradiol were detected. [results] estrogen could induce alkaline phosphatase activity, osteopontin, osteopontin and osteocalcin production in MC3T3-E1 cells. The production of cytokine IL-6 was inhibited after estrogen treatment, and the expression of TGF- 尾 was up-regulated. After treatment with ER 尾 -17 尾 -estradiol, the expression of interleukin 6 and TGF- 尾 mRNA in MC3T3-E1 cells did not change significantly. [conclusion] estrogen regulates the expression of IL-6 and TGF- 尾 through ER 尾, and then regulates the function and bone formation of osteoblasts.
【作者单位】：中南大学湘雅医院脊柱外科湘雅脊柱外科中心;
【基金】：国家自然科学基金资助项目(编号:81271940) 湖南省自然科学基金重点项目(编号:12JJ2043)
【分类号】：R336

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