心肌缺血型冠心病肾虚血瘀病证结合模型研究

发布时间：2018-08-17 19:17

【摘要】：目的探讨手术方法建立心肌缺血型冠心病与肾虚血瘀病证结合模型的可行性。方法采取惊吓、置于寒冷环境并皮下注射氢化可的松的方法制备非疾病肾虚血瘀证候模型,结扎冠状动脉前降支并注射氢化可的松建立心肌缺血型冠心病肾虚血瘀病证结合模型。实验大鼠分为肾虚血瘀组、病证结合组和正常组,每组5只。观察大鼠造模前后体温、体质量、呼吸频率、心率、全血黏度、卡松黏度。依据中医临床肾虚血瘀证诊断标准,对2种模型中医证候进行比较。结果与正常组和本组造模前比较,肾虚血瘀组、病证结合组大鼠造模后均出现体温下降、呼吸频率增快(P0.05);与正常组比较,病证结合组、肾虚血瘀组体质量减轻或者增长缓慢、全血黏度及卡松黏度升高(P0.05,P0.01);造模两组间全血黏度、心电图比较差异有统计学意义(P0.05)。结论 2种模型在中医证候方面无明显差异,符合中医临床肾虚血瘀证的诊断标准。
[Abstract]:Objective to explore the feasibility of establishing a model of myocardial ischemic coronary heart disease combined with kidney deficiency and blood stasis syndrome. Methods the model of non-disease kidney deficiency and blood stasis syndrome was established by shock, cold environment and subcutaneous injection of hydrocortisone. Ligation of anterior descending branch of coronary artery and injection of hydrocortisone were used to establish the model of kidney deficiency and blood stasis syndrome of myocardial ischemia coronary heart disease. Experimental rats were divided into kidney deficiency and blood stasis group, disease combined group and normal group, 5 rats in each group. Body temperature, body mass, respiratory frequency, heart rate, whole blood viscosity and Casson viscosity were observed before and after modeling. According to the diagnostic criteria of kidney deficiency and blood stasis syndrome in traditional Chinese medicine, the TCM syndromes of two kinds of models were compared. Results compared with the normal group and the model group, the body temperature decreased and the respiratory rate increased rapidly in the kidney deficiency and blood stasis group and the disease syndrome combined group (P0.05), compared with the normal group, the body mass of the kidney deficiency and blood stasis group decreased or increased slowly, compared with the normal group. The whole blood viscosity and Casson viscosity increased (P0.05P0.01); the whole blood viscosity and electrocardiogram (ECG) were significantly different between the two groups (P0.05). Conclusion the two models have no significant difference in TCM syndromes and accord with the diagnostic criteria of kidney deficiency and blood stasis syndrome.
【作者单位】：中国中医科学院广安门医院;
【基金】：国家科技支撑计划(2007BAI10B01-093) 北京市自然科学基金(7122153) 北京中医药“薪火传承3+3工程”(830104)
【分类号】：R-332;R259

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