大鼠慢性牙周炎合并动脉粥样硬化动物模型的建立及白细胞介素-6和C反应蛋白表达分析

发布时间：2018-10-15 11:11

【摘要】： 目的:用Wistar大鼠建立动脉粥样硬化(atherosclerosis,As)合并慢性牙周炎(chronic periodontitis,CP)动物模型,以探讨冠心病与慢性牙周炎可能的相关性。方法:60只大鼠随机分为四组,每组15只:A正常对照组,B慢性牙周炎组,C动脉粥样硬化组,D慢性牙周炎合并动脉粥样硬化组。各组接受相应的建模处理。实验周期为12周。每20天检查牙周指标并测定体质量。建模结束后,检测血脂水平及ELISA法检测血清白介素6(interleukin-6,IL-6)、C反应蛋白(C-reactive protein,CRP)水平。组织病理学观察各组实验牙牙周组织及动脉血管病理改变。结果:牙周检查发现,B、D组附着丧失水平(attachment loss,AL)、龈沟出血指数(sulcular bleeding index,SBI)较A、C组有明显增加,且差别有统计学意义(P0.05)。C、D组TC、TG、LDL水平较A、B组明显升高(P0.05),D组血清IL-6、CRP水平均高于其他组(P0.05)。组织病理检查发现B组动脉血管中膜可见不均匀增厚。C、D组主动脉及胸、腹主动脉形成动脉粥样硬化病变。结论:结合牙周检查、血脂检测和组织病理学检查综合判定,初步成功建立大鼠CP合并As动物模型。对各组IL-6、CRP水平的分析,CP可能通过炎症因子的作用影响As的发生发展。
[Abstract]:Aim: to establish an animal model of atherosclerosis (atherosclerosis,As) with chronic periodontitis (chronic periodontitis,CP) in Wistar rats and to explore the possible correlation between coronary heart disease and chronic periodontitis. Methods: sixty rats were randomly divided into four groups, 15 rats in each group: a control group, B chronic periodontitis group, C atherosclerosis group, D chronic periodontitis combined with atherosclerosis group. Each group was treated with corresponding modeling. The experimental period was 12 weeks. Periodontal indexes were examined and body mass was measured every 20 days. After modeling, serum lipid level and serum interleukin 6 (interleukin-6,IL-6), C reactive protein) (C-reactive protein,CRP) level were detected by ELISA method. Histopathological changes of periodontal tissues and arterial vessels of experimental teeth were observed. Results: periodontal examination showed that the level of attachment loss (attachment loss,AL) and gingival sulcus bleeding index (sulcular bleeding index,SBI) in group B were significantly higher than those in group A (P 0.05), and the TC,TG,LDL level in group C was significantly higher than that in group A (P0.05). The level of serum IL-6,CRP in group C was significantly higher than that in group A (P0.05). Histopathological examination showed that there was an uneven thickening of the medial membrane of the arteries in group B. Atherosclerosis was found in the aorta, thoracic and abdominal aorta in group C and D. Conclusion: combined with periodontal examination, blood lipid detection and histopathological examination, the animal model of CP combined with As was established successfully. The level of IL-6,CRP in each group was analyzed. CP may influence the development of As through the action of inflammatory factors.
【学位授予单位】：新疆医科大学
【学位级别】：硕士
【学位授予年份】：2009
【分类号】：R781.4;R543.5;R-332

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