去卵巢及雌激素替代治疗后家兔边缘系统NOS的活性及nNOS阳性神经元的形态结构与分布变化

发布时间：2018-10-17 22:26

【摘要】： 为了探讨雌激素、孕激素对去卵巢家兔边缘系统NOS的活性及对nNOS阳性神经元形态结构分布的影响,本试验将家兔切除卵巢,作为妇女更年期后卵巢功能衰竭、雌激素分泌严重不足时的良好模型,然后通过人为注射雌激素及雌激素和孕激素,观察去卵巢和注射雌激素及雌激素和孕激素后家兔血清中NO的浓度、边缘系统NOS活性及nNOS阳性神经元的形态结构分布变化来研究雌激素保护中枢神经系统、预防老年痴呆和记忆力减退等神经系疾病的机理,为神经生理学和病理学研究提供形态学依据。利用SABC免疫组织化学法,在光学显微镜下对各组家兔海马CA1、CA2、CA3区及齿状回、下丘脑室旁核、下丘脑外侧区、视上核以及额叶神经型一氧化氮合酶(nNOS)阳性神经元的形态结构及分布进行观测。结果表明:假手术对照组家兔海马、下丘脑、额叶内有大量的nNOS阳性神经元分布;去卵巢后nNOS阳性神经元形态分布变化有区域差异,额叶和海马各区(除CA2外)nNOS阳性神经元表达明显减弱,下丘脑各神经核nNOS阳性神经元的表达明显增强,即数量增多,染色加深;雌激素替代治疗组与假手术组之间无显著差异,额叶和海马各区(除CA2外)nNOS阳性神经元表达明显增强,下丘脑各神经核nNOS阳性神经元的表达明显减弱,即数量减少,染色变浅。利用硝酸还原酶法和生物化学检测技术,采用NO、NOS试剂盒分别检测去卵巢和注射雌激素及雌激素和孕激素后家兔血清中NO的浓度的变化及大脑边缘系统NOS的活性变化。结果表明:去卵巢三个月后家兔血清NO水平和大脑边缘系统NOS活性降低;单纯补充雌激素和联合使用雌激素、孕激素时,血清NO水平和大脑边缘系统NOS活性均升高;单纯补充雌激素血清NO水平和大脑边缘系统NOS的活性高于联合使用雌激素、孕激素时血清NO水平和大脑边缘系统NOS活性,但差异不显著。
[Abstract]:In order to investigate the effects of estrogen and progesterone on the activity of NOS in the limbic system of ovariectomized rabbits and on the morphological and structural distribution of nNOS positive neurons, the ovariectomized rabbits were treated as ovarian failure after menopause. A good model when estrogen secretion was seriously deficient, then the serum NO concentration of rabbits after ovariectomized and injected with estrogen and progesterone was observed by artificial injection of estrogen, estrogen and progesterone. In order to study the mechanism of estrogen protecting the central nervous system and preventing senile dementia and memory loss, the activity of NOS in the limbic system and the morphological and structural changes of nNOS positive neurons were studied. To provide morphological basis for neurophysiology and pathology. Using SABC immunohistochemical method, the CA1,CA2,CA3 area, dentate gyrus, paraventricular nucleus of hypothalamus and lateral area of hypothalamus in each group were studied under optical microscope. The morphology and distribution of neuronal nitric oxide synthase (nNOS) positive neurons in supraoptic nucleus and frontal lobe were observed. The results showed that there were a large number of nNOS positive neurons in hippocampus, hypothalamus and frontal lobe of rabbits in sham operation control group, and there were regional differences in morphological distribution of nNOS positive neurons after ovariectomy. The expression of nNOS positive neurons in frontal lobe and hippocampal regions (except CA2) was significantly decreased, and the expression of nNOS positive neurons in hypothalamic nuclei was significantly increased, that is, the number of nNOS positive neurons increased and the staining deepened, there was no significant difference between estrogen replacement therapy group and sham-operation group. The expression of nNOS positive neurons in frontal lobe and hippocampal regions (except CA2) was significantly increased, and the expression of nNOS positive neurons in hypothalamic nuclei was significantly decreased, that is, the number of nNOS positive neurons decreased and the staining became shallower. NO,NOS kit was used to detect the changes of serum NO concentration and the activity of NOS in brain limbic system of rabbits after ovariectomized and injected estrogen and progesterone with nitrate reductase and biochemical techniques. The results showed that after ovariectomized for 3 months, the level of serum NO and the activity of NOS in the cerebrum limbic system were decreased, and the level of serum NO and the activity of NOS in the limbic system of the brain were increased after estrogen supplementation and combined use of progesterone. The level of serum NO and the activity of NOS in the limbic system of brain were higher than those in the combination of estrogen and progesterone, but there was no significant difference in the level of serum NO and the activity of NOS in the limbic system of brain with progesterone.
【学位授予单位】：山东农业大学
【学位级别】：硕士
【学位授予年份】：2010
【分类号】：R-332;R361.2

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