基于mtDNA和rDNA基因序列的我国按蚊属按蚊亚属系统发育研究

发布时间：2018-11-16 17:13

【摘要】：目的应用mt DNA和r DNA基因特征重建我国按蚊属按蚊亚属的系统发育关系,以阐明各蚊种的亲缘关系。方法测定和分析我国按蚊属按蚊亚属17种蚊的mt DNA-COI和COII,以及r DNA-28S-D3基因序列,采用单基因和联合基因数据组以邻接法(NJ)、最大似然法(ML)和最小距离法(ME)构建系统发育树。结果 mt DNA-COI和COII基因,以及r DNA-28S-D3序列的长度分别为605bp、685 bp和370bp~406 bp。COI和COII在按蚊亚属成对蚊种间的遗传距离分别为0.002~0.111和0.001~0.105;而D3序列在多个蚊种间无差异,最大遗传距离为0.163。基于单基因COI和联合数据组"COI+D3"、"COI+COII+D3"构建的系统树拓扑结构一致、分支合理且稳定。各种团成员首先聚类,各大支分别对应4个种团,其间林氏按蚊种团与赫坎按蚊种团亲缘关系最近,须喙按蚊种团与之较远。结论构建的中国按蚊属按蚊亚属的分子系统发育树客观地阐释了各蚊种之间的亲缘关系,mt DNA-COI基因序列和多基因联合具足够的信息贡献量。
[Abstract]:Objective to reconstruct the phylogenetic relationship of Anopheles subgenus Anopheles by using mt DNA and r DNA gene characteristics in order to elucidate the genetic relationship among different species of Anopheles in China. Methods the sequences of mt DNA-COI, COII, and r DNA-28S-D3 genes of 17 species of Anopheles subgenus in China were determined and analyzed. Single gene and combined gene data sets were used to detect and analyze the sequence of (NJ),. Phylogenetic tree was constructed by maximum likelihood method (ML) and minimum distance method (ME). Results the length of mt DNA-COI and COII genes and r DNA-28S-D3 sequences were 605bpn685 bp and 370bp~406 bp.COI and COII, respectively. The genetic distances between Anopheles subgenus and Anopheles japonicus were 0.002 (0.111) and 0.001 (0.105), respectively. The maximum genetic distance of D3 sequence was 0.163. The system tree based on single gene COI and combined data set "COI D3" and "COI COII D3" has the same topological structure, reasonable and stable branches. At first, the members of each group were clustered, each branch corresponding to four species groups respectively. During this period, the relationship between Anopheles Linsoni and Anopheles hekanensis was close, and the Anopheles punctatus group was far away from the Anopheles albopictus. Conclusion the molecular phylogenetic tree of Anopheles subgenus Anopheles in China objectively explains the genetic relationship among mosquito species, and the combination of mt DNA-COI gene sequence and polygene has sufficient information contribution.
【作者单位】：第二军医大学热带传染病学教研室;
【基金】：国家自然科学基金委员会-云南省人民政府联合基金(No.U0932604)
【分类号】：R384.1

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