深圳地区2014年柯萨奇病毒A组4型VP1区基因特征分析

发布时间：2019-01-06 07:18

【摘要】：目的对2014年深圳市罗湖区一起疱疹性咽峡炎疫情的病原体进行鉴定,并对鉴定的柯萨奇A4病毒(CVA4)的VP1基因进行序列测定和遗传进化特征分析。方法采集疫情中患儿的咽拭子样本8份,提取病毒核酸,利用荧光RTPCR法检测样本总肠道病毒(EV)、人肠道病毒71型(EV71)、柯萨奇病毒A组16型(CVAl6)、CVA4、CVA6和CVAl0等,对CVA4阳性样本采用RT-PCR方法扩增其VP1区全长序列,并进行核苷酸序列测定和遗传进化分析。结果引起此次疱疹性咽峡炎暴发的病原体为GIB亚型的CVA4病毒。同源性分析显示,深圳2014年CVA4病毒株与云南2004年分离株(AB268278)、以及台湾2008年分离株(AB571570)核苷酸同源性达94.1%~94.8%,而与CVA4原型株HIGHPOINT同源性最低,为84.3%。在氨基酸序列上与台湾2006年分离株(AB571563)、吉林2006年分离株(JQ715709)同源性最高,达99.3%;而与山东省2006年分离株(GQ253375)同源性最低,为97.1%。相比深圳2009年分离株(HQ728260),共有6个氨基酸突变位点:N22S,T34A,N63S,A165D,T200A和V126A;而进化树分析显示,深圳2014年CVA4病毒株虽属于CVA4的GIB亚型,但在进化走势上,已经不同于GIB亚型中其他地区早期的分离株。结论 2014年深圳地区CVA4病毒属于GIB亚型,但在VP1区变异较大。
[Abstract]:Objective to identify the pathogen of a herpetic pharyngitis epidemic in Luohu District, Shenzhen City in 2014, and to sequence the VP1 gene of Coxsackie A4 virus (CVA4) and analyze its genetic and evolutionary characteristics. Methods eight throat swabs of children were collected and virus nucleic acids were extracted. Total enterovirus (EV), human enterovirus 71 (EV71), coxsackie virus A group 16 (CVAl6), CVA4,CVA6 and CVAl0 were detected by fluorescence RTPCR assay. The full-length VP1 region of CVA4 positive samples was amplified by RT-PCR, and the nucleotide sequence was determined and genetic evolution was analyzed. Results the pathogen causing the outbreak of herpes isthmus was CVA4 virus of GIB subtype. Homology analysis showed that the nucleotide homology between Shenzhen 2014 CVA4 virus strain and Yunnan 2004 strain (AB268278) and Taiwan 2008 strain (AB571570) was 94.1% and 94.8% respectively, but lowest with CVA4 prototype strain HIGHPOINT (84.3%). The amino acid sequence was the highest (99.3) homology with Taiwan 2006 (AB571563), Jilin 2006 (JQ715709), and Shandong province 2006 (GQ253375) with the lowest homology (97.1%). Compared with Shenzhen strain (HQ728260) in 2009, there were 6 amino acid mutation loci: N22Stit T34A, N63SN A165DN T200A and V126A; The phylogenetic tree analysis showed that the Shenzhen 2014 CVA4 strain belonged to the GIB subtype of CVA4, but the evolutionary trend was different from that of the early isolates in other regions of GIB subtype. Conclusion the CVA4 virus belongs to GIB subtype in Shenzhen area in 2014, but it has great variation in VP1 region.
【作者单位】：深圳市疾病预防控制中心重大传染病监控实验室;深圳市罗湖区疾病预防控制中心微生物检验科;
【基金】：广东省医学科研基金(No.A2013602)~~
【分类号】：R373

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