人口腔疣状癌裸鼠移植瘤模型的建立及Survivin和Bcl-2的表达分析

发布时间：2019-05-05 09:10

【摘要】： 目的:对口腔疣状癌的裸鼠建模进行初步的实验研究,为深入研究口腔疣状癌的发生、发展及其演变机理提供有益的探索模型。材料与方法:将口腔疣状癌癌组织采用组织块移植法和细胞悬液注射法移植于Balb/c裸鼠皮下,建立人口腔疣状癌裸鼠移植瘤模型(模型一);将复发性疣状癌伴鳞癌颈部转移灶采用组织块移植法移植于裸鼠右后背部皮下,建立人复发性疣状癌术后伴鳞癌颈淋巴转移裸鼠移植瘤模型(模型二)。观察移植瘤生长特性,计算移植成功率,绘制生长曲线,并对移植瘤进行病理学和组织学检查。结果:模型一建模失败,模型二原代移植于8只裸鼠有7只获得成功,成瘤率87.5%(7/8),肿瘤生长曲线符合Gompertz函数曲线的一般规律。移植瘤最后都出现坏死,个别还出现消退现象。皮下肿瘤瘤体呈圆球形或椭圆形,表面光滑,包膜完整,呈结节状突起,与周围组织界限清楚,活动度良好,有的肿瘤呈向心性坏死,有的肿瘤表面可见清晰的毛细血管,表面肉红色,剖面呈鱼肉状,质韧,有的呈白色豆渣状。光镜下移植瘤细胞成多角形,异型性明显,核浆比例增大。核椭圆形、圆形、大小不等,核分裂相多见,并见病理核分裂。癌细胞排列呈巢状、片状或条索状,其间可见角化珠。肝、肺、肾未见转移,前肢腋下及腹股沟淋巴结反应性增生。移植瘤中Survivin,Bcl-2蛋白免疫组织化学染色和人肿瘤比较差异无显著性(P＞0.05)。结论: 1.建立人复发性疣状癌术后伴鳞癌颈淋巴结转移裸鼠原代移植瘤模型获得成功,为下一步建立稳定的移植瘤模型奠定了坚实的基础。 2.人复发性疣状癌伴鳞癌颈淋巴转移原代移植瘤保留了人鳞癌的生物学特点,并与原发肿瘤的组织学特征相似。 3.Survivin和Bcl-2在移植瘤及人高分化鳞癌中的表达差异无显著性。
[Abstract]:Objective: To study the model of oral cavity carcinoma in nude mice, and to provide a useful exploration model for the study of the occurrence, development and evolution mechanism of oral cavity carcinoma. Materials and Methods: The tissue-block transplantation method and cell suspension injection method were applied to the subcutaneous of Balb/ c nude mice. 1). The cervical lymph node metastasis of recurrent cervical carcinoma with squamous cell carcinoma was transplanted to the right back of nude mice by a tissue-block transplantation method, and a model of the tumor-associated cervical lymph node metastasis in nude mice (model) was established. 2) observing the growth characteristics of the transplanted tumor, calculating the success rate of the transplantation, drawing the growth curve, and carrying out pathology and histology on the transplanted tumor. Results: The model one model failed, the model 2 was transplanted to 8 nude mice with 7 success, the tumor rate was 87.5% (7/8), and the tumor growth curve was in line with the Gompertz function curve. The general rule of the tumor is that the transplanted tumor finally has necrosis, and the individual also The tumor of the subcutaneous tumor is spherical or elliptical, the surface is smooth, the envelope is complete, the nodular protrusion is in the shape of a nodular, the boundary of the surrounding tissue is clear, the activity is good, the tumor is in the heart-like necrosis, the surface of the tumor is visible and clear, and the surface meat is red and cut. The surface is fish-like, tough, and has the shape of white bean dregs-shaped. The transplanted tumor cells under the light microscope are polygonal, the heterotypic nature is obvious, the nucleus The proportion of the pulp is increased. The kernel is elliptical, circular, different in size, and the nuclear fission is more common. See also The cancer cells are arranged in the form of a nest, a sheet, or a strip. visible keratosis, no transfer of liver, lung, and kidney, subaxillary and inguinal lymph nodes of the forepart The expression of Survivin and Bcl-2 in the transplanted tumor was not significant (P> 0.0 5). Conclusion:1. The successful development of primary transplanted tumor model of the nude mice with squamous cell carcinoma with cervical lymph node metastasis after the operation of recurrent papillary carcinoma of human is established, and a stable transplantation is established for the next step. the tumor model lays a solid foundation.2. The primary transplant tumor of the human recurrent papillary carcinoma with the cervical lymph node metastasis of the squamous cell carcinoma retains the biological characteristics of the human squamous cell carcinoma, And the histological features of the primary tumor were similar to those of the primary tumor.
【学位授予单位】：中南大学
【学位级别】：硕士
【学位授予年份】：2008
【分类号】：R739.8;R-332

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